Does anyone know what happened to these fish embryo samples in 4% PFA in PBS?

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Tulay Irez

You have to fix it after zona drilling

Jihye Yoon

Thank you for your answering.

I usually been sampled embryos from far field.

So I collect lots of eggs once in 4% PFA without dechorion treatment.

After then, I remove the chorion and fix it 4% PFA again for 12 hr.

Is it impossible way to WISH?