Jihye Yoon

2 Questions 3 Answers 0 Followers

Questions related from Jihye Yoon

Does anyone know what happened to these fish embryo samples in 4% PFA in PBS?

I'm trying to whole mount in situ hybridization in fish embryos. I fixed fish embryos in 4% PFA in PBS for 2days. And I found some sharp brown spots in embryo. I've never found that spots...

13 January 2021 2,840 2 View

Is there any solutions to prevent to be torn gastrula stage embryo during whole mount in situ hybridization?

Hi. Everyone. I'm trying to Whole mount in situ hybridization. In gastrula stage, cells were easily be torn even I didn't treat the pk solution. And what is the best way to preserve the...

13 January 2021 9,752 2 View