Recently, I read article by E. Thomas, I. Rathore, and J. C. Tarafdar “Bio-Inspired Synthesis of Nitrogen Nanoparticles and Its Application on Pearl Millet (Pennisetum americanum L) cv. HHB 67”. The present work demonstrated the biosynthesis of the Nitrogen nanoparticles after isolating and identifying an efficient soil fungi Aspergillus tubingenesis. The fungus was found to be very efficient and producing very tiny nanoparticles (1–2 nm size) from NH4NO3 salts. The doses for application to plants were also optimized as 80 ppm. The results suggest that 80 ppm concentration of nano-N application producing by our developed fungus was found to be safer limit and help in significant improvement in root area, length, perimeter, number of tips, root diameter, beneficial enzymes and microbial activities as well as total dry biomass.

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