Can you use primers that are specific for cDNA on DNA?

08 September 2015 6,114 9 View

Can anyone help with extracting DNA from nasal swabs using the TeNP40 method?

11 December 2014 2,132 1 View

What is the most reliable DNA extraction method for clinical samples such as aspirates?

We just got some aspirates from the hospital and plan on extracting DNA for next generation sequencing. I was wondering if anyone who has worked with aspirates before knows which DNA extraction...

10 November 2013 2,091 1 View

What are good positive controls for cytokines IL-10, IL-12p40, IL-17a, Muc-2, and TGF-beta?

I am looking at cytokine gene expression of IL-10, IL-12p40, IL-17a, Muc-2, and TGF-beta in infant stool samples at 12 weeks of time point. I am looking for a good positive control to use to...

04 May 2013 1,783 6 View

What do you recommend, CaCl2 or CaCl2.2H20 for ELISAs?

I need to make an extraction buffer for my stool samples used for ELISAs. Calcium Chloride was one of the ingredients found in most literature for the extraction buffer. However, the type of...

11 December 2012 9,197 2 View

How do you culture PMN? Any suggestion for storage of PMN's?

I just received blood from a patient. I plan on isolating PMN from them. I don't think I will be doing experiments using PMN that same day I isolate it. So I was wondering if PMN can be...

10 November 2012 1,201 7 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View