In my lab, we use ROTEM trombelastometry , it is modification of traditional thromboelastography. It is a test that explores the entire process of coagulation global and comprehensively, from the primary hemostasis and secondary to fibrinolysis. It is a device patented by Pentapharm GMBH, Munich, measuring tromboelastometria. The basic principle consists of a stroke that occurs when measuring the polymerization of fibrinaLa-associated changes in the elasticidad-viscosidad of the clot blood sample is placed in a bucket. A cylindrical pin subject to an axis is immersed in it. The movements of rotation among them are detected and sent to a computer. Coagulation and Lysis changes of torque which also detects the computer and are reflected in the printed graphics along with the corresponding numeric parameters. Parameters - Clotting (CT) time: time from the beginning of the measurement until the start of the clot. It is measured in seconds. It gives us information about plasma coagulation or the existence of circulating anticoagulant factors. -Time of formation of the clot (CFT): time from the beginning of the clot until it detects a firmness of 20 mm. It is measured in seconds and gives information about factors, anticoagulants, fibrin polymerization and stabilization of the clot with platelets, fibrin and factor XIII.-maximum firmness of the clot (MCF): is the maximum amplitude of the curve. It is measured in millimeters. Valued Fibrinogen and platelet factor XIII.-maximum Lysis (ML): reduction of the firmness of the clot after the MCF. The clot remains stable if ML < 15%. starts to occur fibrinolysis if ML > 15%. Measured at the 30 and 60 minutes. Test - EXTEM: Global Test that analyzes the extrinsic route, from the coagulation cascade until the consolidation of the clot and subsequent fibrinolysis. Corresponds to the time of prothrombin-outside: global Test that analyzes the intrinsic route, from the cascade of clotting until the consolidation of the clot. Corresponds to the partial thromboplastin time-activated-FIBTEM: specific monitoring of Fibrinogen-APTEM: it resembles the EXTEM, but reagent contains aprotinin, which inhibits fibrinolysis-HEPTEM: it is similar to the outside, but the reagent contains heparinase and detectable alterations in coagulation which are due to heparin. Advantages - faster treatment, oriented and guided-saving blood products.-better patient care by minor blood loss.-reduced hospital stay time.-greater control of the consumption of F VII, Fibrinogen, f. VIII, XIII - monitor situations of hypercoagulability is used: - at the beginning of the surgery-if you see a striking bleeding - after each therapeutic action with blood products or complex procoagulant.-at the end of the procedure.-in the postoperative period.
In our department, we use ROTEM system. So far we are content with performance in evaluation of global hemostasis as well as fibrinogen specific monitoring and basic prameters as described above by Juan Carlos. We monitor haemostasis in pregnant patients and post delivery as well as perioperational monitoring and fibrinogen disorders such as dys- and afibrinogenemia.