How to calculate visual angle of a visual stimulus?

I am currently trying to figure out how to calculate the visual angle of some visual stimuli I used in an experiment. I think I have all of the information I need to calculate this. One is a...

15 February 2021 3,189 2 View

Having trouble with qPCR amplification plots. My curve's never seem to reach a plateau. Any ideas?

The parameters of the curve all seem acceptable, typically reactions results are above 0.9852, -3.1 to -3.6 slope, and 90-110% reaction efficiency. The results seem to be what I would expect from...

04 February 2021 1,138 3 View

How to make sure self-ligation of a linear plasmid?

I want to ligate a linear 1.7kbp plasmid with the EcoRI sites at two sides, but when I use the quick ligase kit from NEB to test the ligation efficiency it is hard to ligate it. Is there anyone...

08 December 2020 3,442 3 View

What effect does light have on the SLS plant tissue decelluarization process?

does it speed it up?

29 November 2020 6,168 3 View

Significant degradation of RNA in RNAlater storage at 4C for longer than a month?

I misread the amount of time that RNA was stable in RNA later at 4 C as several months instead of a month. I have e zebrafish samples that have taken me a 2 months to collect and they have been...

23 October 2020 8,085 3 View

How to present the results of a binary logistic regression for an ecological paper?

I conducted a binary logistic regression using the glm function in R. My binary predictor is the presence or absence of my study species. I have two significant variables but am just not sure what...

19 October 2020 5,398 3 View

Basic question - how to make a cell dissociation buffer?

Dear All, I'm looking to make a cell dissociation buffer from lyophilised collagen I (Gibco, 12648010). The protocols I've been looking at all specify concentrations of enzymes in terms of...

06 October 2020 506 3 View

What is the best way to perform a serial dilution of E.coli, when the culture is starting on a solid media (LB)?

I have an LB growth media plate with E.coli on it. I want to dilute the culture. I am working with 3M Coliform detection plates, and I want to get a solution of E.coli diluted enough to grow...

27 August 2020 3,043 3 View

Why fluorescence signal drops during thermal shift assay?

I am performing a TSA to investigate binding of a ligand to the protein by performing a serial dilution of ligand against same conc. of protein (~2uM). Interestingly at low ligand conc the...

02 August 2020 3,167 1 View

Writing specific contact surface behaviour using Userinter.F in ANSYS?

Hello, Is there anybody who has experience developing any user subroutine using Userinter.F for contact simulations in ANSYS? Any advise and example would be appreciated highly. thanks a lot

21 February 2020 6,495 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View