Please do not suggest aCamkII - I tried it, it works... kind of... not really good signal-to-noise ratio. I am looking for something that gives a signal similar to GAD67 immunofluorescence.
Hi Everyone, I am working on a research project focused on analyzing behavior and cognition in different environments and situations. I am looking for a solution that allows us to: Record...
29 June 2024 6,540 0 View
Does anyone know an unbiased way to define the cavity and volume of macrocycles, such as crown ethers, cyclodextrin, and similar molecules, using quantum chemistry software? All papers that I'm...
25 March 2024 5,265 8 View
Cities compete with each other in a globalised environment. How does this affect the design of urban space and the community?
04 March 2024 3,319 2 View
Hi, I need to do a co-culture with these two cell types and I would like to do a qPCR. Unfortunately I can't do a flow cytometry so I would use a magnetic beads separation system to isolate the...
19 February 2024 7,155 0 View
I have FTIR spectra and I need to calculate their FWHM. I tried many times on Origin, but never worked. I guess that is something related to the old version that I am using (8.5). Can you help...
09 February 2024 4,241 7 View
I have read about the topic and I was wondering if anybody could help me to interpret the following description of repeated measurements I found in a manuscript (very detailed but still confuse in...
14 December 2023 4,028 3 View
I have bi-weekly UAV flights in soybean trials and I was wondering about removing or not pixels classified as "soil" from the images to calculate veg. indices. Initially, I am calculating for of...
13 November 2023 1,838 1 View
I am calculating the gruneisen parameter for a structure with 28 atoms in a 1x1x1 supercell and 224 in a 2x2x2 supercell, and I am having some issues with phonopy. When I try to run the...
10 October 2023 2,070 2 View
I am running a phylogenetic logistic regression using brm from brms package. My data consists on a categorical predictor (values 0, 1 and 2) and a binary response variabe (values 1 and 2) plus...
06 August 2023 2,998 1 View
I am currently exploring advanced mathematical techniques within the field of econometrics and am particularly interested in understanding how third-order differential equations, commonly referred...
02 August 2023 9,473 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View