Does anyone know of an efficient way to selectively stain (immunofluorescence) the soma of excitatory cells?

11 November 2013 0 5K Report

Please do not suggest aCamkII - I tried it, it works... kind of... not really good signal-to-noise ratio. I am looking for something that gives a signal similar to GAD67 immunofluorescence.

Badges
Science topic

Similar topics
Geoscience
Cartography
Mapping

More Leonardo Restivo's questions See All

No title

They are software's that are used mainly in the discipline of psychology

05 August 2020 9,423 3 View

Which value of volume fraction I can to choose for to identify free surface in an air-water simulation of open channel?

In air-water multiphase flow CFD simulation's it is common to use a VOF = 0.5 to track or identify the position of interface or free surface. I would like to know why this criteria is used and if...

12 July 2020 7,793 9 View

How to decide if to get 1 or various P value? regarding an composting process through time and treatments?

I have a research investigation that I got the results, I applied 4 different composting processes to vegetable waste (manure, manure+Effective Microorganisms, Effective Microorganisms and a...

14 June 2020 2,373 2 View

What is a CFD and other question?

To me, a CFD is solve a fluid mechanics problem using mathematical approaches with the help of a computer. In other words, it is like a marriage between a mathematical model and numerical methods...

04 May 2020 2,811 25 View

How to analyse the results of an alloy XRD?

Having studied Elements of XRD book and indexed an alloy profile, it remains some doubts, some of them are ordinary because the lack of knowledge of mine. I would kindly ask someone to explain or...

24 April 2020 7,893 6 View

Purification of histag protein using HISTRAP HP (GE). What is the best lysis buffer and which protocol may be most suitable?

Purification of histag protein using HISTRAP HP (GE). I am working with insect cells (Tn5B) and my antigens are expressed in the nucleus. I am having difficulty lysing the cells because we have no...

10 March 2020 8,609 2 View

Recent meta-analysis on imaging treatment response predictors in depression?

Dear Experts, Would anyone know of any recent meta-analysis of neuroimaging predictors of treatment response in major depression? I have looked around and all I could find was a paper from 8...

27 January 2020 9,717 1 View

Any suggestion for the amplification of Wingless in old collection samples?

Hello to everyone, I'm trying to amplify nuclear markers from old museum specimens of beetle (not very old, sampled 10-15 years ago) , I thought on the Wingless fragment, it's obviously a hard...

02 December 2019 7,463 3 View

How to reduce massive protein degradation during purification?

Dear all, I am expressing in BL21(DE3) E. coli cells and subsequently purifying a 6x-His-tagged 50 kDa truncated protein which looks like to be very unstable. Its domains are literally falling...

17 October 2019 1,416 13 View

Why p-values are higher when I run a logistic regression with all variables together, but significant when I do separately?

I ran an experiment with a 2x2 design, like the dependent variable I have a dichotomic variable. When I running a factorial ANOVA, interaction is significant and one principal effect, also when I...

14 October 2019 9,342 6 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View