We did it with HRVs (JMS 2001;36(9):1038-52; Particuology 2013,11:14-19).

Re. bacteria try to contact S. Grinshpun (http://www.eh.uc.edu/aerosol/Grinshpun%20main.htm). He does work with bacteria.

Isothermal microcalorimetry does not care about the physical form of the sample (i.e., gas, liquid, or solid). We have made measurement of bacteria growing within solids and liquids... maybe it can be a lead for your measurements. If you give a little bit more specific details I can give you a more specific answer on the feasibility. In the meantime you can take a look at the following:

http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2009.01819.x/abstract

http://www.sciencedirect.com/science/article/pii/S0040603112005813

http://onlinelibrary.wiley.com/doi/10.1111/1574-6968.12007/abstract

Check this paper by Pyrgiotakis et al

Article citation: Environ. Sci.: Nano, 2014, 1 (1), 15 - 26

Title: A Chemical free, Nanotechnology-based Method for Airborne Bacterial Inactivation using Engineered Water Nanostructures

You can use live/dead kit to detect viable bacteria directly on black filter in different times using epifluorescent microscope

Several years ago, I was involved in a project to measure viable aersolized microorganisms (bacteria and enteric virus) around a sewage treatment plant. We employed three different sampling devices, one an Anderson Sampler that segregated bacterial aerosols by size, a second, called a "slit sampler" which measured total bacteria over time (usually set to 1 hour). The third was used to collect samples in a volume of fluid where the air was sucked into an enclosed conical glass "scrubber" and it would then impinge onto the back of the glass as the collection media flowed down into a collecting vessel thereby collecting any viable aerosols that were present. The first two utilized TSA non-selective media. The scrubber fluid was concentrated and analyzed on selective media for fecal-related bacteria and for enteric viruses. This technology grew out of germ warfare work that was done by the DOD back in the 60's and 70's.

You can refer the Lokabharathi et al. 1976 where they have counted the viable counts in sea water. you can collect aerosols in liquid media and filter on membrane filter and counting of viable bacteria can be done two ways one fluorescent method using DAPI and another one haemocytometer method. if use media for the same then you may get only culturable fraction but there are many uncultured ones.

Real-time microscopy video with viability staining?

This paper was posted recently at the Journal of Aerosol Science:

A Systematic Comparison of Four Bioaerosol Generators: Affect on Culturability and Cell Membrane Integrity when Aerosolizing Escherichia coli Bacteria

Huajun Zhen, Taewon Han, Donna E. Fennell, and Gediminas Mainelis*

Rutgers University, Department of Environmental Sciences, 14 College Farm Rd., New

Brunswick, NJ 08901;

Journal of Aerosol Science

January 2014

DOI: http://dx.doi.org/10.1016/j.jaerosci.2014.01.002

*Corresponding author: Gediminas Mainelis

Phone: 848-932-5712

Fax: 732-932-8644

E-mail: [email protected]

We have some unublished results...If You have find something published, please, forvard it to me! Thank You in advance!

You can use the e-mail:

[email protected]

Thank you all for your feedback and comments.