Does anyone know if induced neurons can generated from a stem cell-like state?

More Nafiseh Sanei ata-abadi's questions See All

Is there any machine to do real time pcr?

I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering

05 August 2024 1,660 0 View

What method is suitable to use for small sample in panel data T=15 N=8?

i want to know; that how to proceed with the mentioned data set to capture the long run and short run association in panel data, thank you

15 May 2024 6,946 6 View

What is the best way to couple light from integrating sphere into a multimode fibre?

I am setting up a test to measure power and spectrum of laser dies. To avoid precise alignment, and also due to lasers' high output power, I have decided to use an integrating sphere to do the...

31 March 2024 4,170 6 View

What is the best software for Meta-analysis?

What is the best software for meta-analysis? I usually use RevMan for meta-analysis. What is the limitation of RevMan compared to other software (STATA or CMA)?

16 December 2023 6,419 5 View

Do you have a solution for making CO2 in the incubator?

In our cell culture lab, the incubator has a problem with making CO2, and all our cells are in there. Do you have any idea to make CO2 in the incubator or any idea about protecting our cells while...

25 November 2023 8,289 3 View

From which reference can I download example CTD for drug or antivenom?

CTD Form or CTD example

27 October 2023 3,358 0 View

Hello Has anyone worked on stem cells and has any skills? How can I start my studies in this field?

cancer stem cell, use for treatment

12 October 2023 6,045 2 View

Steralisation method of (1→3)-β-D-glucans ?

Dear: I would like to prepare a sterile solution for injection of animals under my experiment with 1,3-ᴅ-glucan. but it was recorded that, steam autoclave affects the criteria of the extract. So,...

24 September 2023 430 0 View

What dye is better to use in confocal laser scanning microscopy for protein analysis in extracted starches?

Looking for technical guide on better dye to stain protein in starch samples for study unders confocal laser scanning microscopy.

09 September 2023 5,442 1 View

How does predictor importance calculate in SPSS Modeler?

Hi! How does predictor importance calculate in SPSS Modeler? ( It is mentioned with the help of SW that the predictor importance is relative, So, it is not based on OR?) In different models of...

28 August 2023 5,526 2 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View