I read a paper where the heat stability of thylakoid membranes and loss chlorophyll in heat stress in winter wheat has a strong correlation. I was wondering if I could use the SPAD to screen several accessions of quinoa in my heat experiments. Because, if I use the chlorophyll fluorescence I will take me long time, due to the leaf needs dark adaptation.
Clearly, chlorophyll fluorescence is more reliable indicator in this situation. Dark adaptation only takes 20 min, and one can use a whole batch of leaf clips for that purpose, so, the delay would not be considerable. SPAD measurement also takes some time...
Yes you can use SPAD to measure chlorophyll contents, but it will only preliminary information about chlorophyll loss under heat stress.
Thanks everyone for your answer, I was thinking to measure the SPAD measurement in my first screening accessions. I hope to collect around 200 different accessions, also I can screen with both methods the chlorophyll fluorescence and the the chlorophyll loss to see correlations. If you know more information about that, please let me know.
Greetings,
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