At the presence of exogenous beta-2-microglobulin, presentation of MHC class I restricted peptides will be much better. Partially, this is due to capability of "empty" MHC class I heavy chains on the cell surface to be refolded at the presence of beta-2-m and peptide. I would recommend to see following references:

https://www.researchgate.net/publication/21553858_Peptide_and_beta_2-microglobulin_regulation_of_cell_surface_MHC_class_I_conformation_and_expression?ev=contentfeed

http://www.sciencemag.org/content/250/4986/1423.abstract

Article Peptide and β2-microglobulin regulation of cell surface MHC ...

Here is additional reference:

http://www.jimmunol.org/content/150/4/1244.abstract

thank you very much for your explanation, however do you have some experience about the nanoparticle pulsed dendritic cells. In my result nanoparticle can dramatically enhance the uptake of the loaded peptide.

Dear Yuan Qian, obviously, I deal with more traditional forms of the antigens, such as allogeneic and microbial cells, proteins, etc. Also, I worked on dendrimeric peptides, which represented tetramers of different MHC class I molecule fragments. At some extent, these examples may be classified under common term "nano". There is a great variety of different synthetic nanoparticles now. So, I have no idea, how much somebody's experience may be helpful in anyone's research. I can not understand, what do you mean under "uptake of the loaded peptide"? Do you want to say, that composition of your nanoparticles stimulate their phagocytosis?

Hi,Dmitry Kazansky ，thanks for your patience, I mean that the peptide was loaded on the nanoparticles co-valently.