Hello Johannes

Thank you very much for the info and the useful links to protocols!

I will try in acetone (I already have it), thanks

Regards

Paula

Hi Paula,

when dissolving the FDA in acetone keep in mind to dilute the FDA stock approximately 1:1000. Otherwise acetone may damage the pollen grain membranes. Usually 2 mg FDA are dissolved per ml acetone. The stock solution can be stored at -20 °C for a few months. Add 1 µl FDA stock per ml of pollen medium and use the staining solution within half an hour to avoid precipitation of FDA.

However, the best test for pollen viability is to germinate them in germination buffer!

Have fun with the experiments

Gerhard

Hi Gerard

Thanks a lot for your advices!!. Do you prefer dissolve FDA in DMSO vs acetone? 

Actually this experiment is complementary to the traditional experiment of pollen germination.

Kind regards

Paula

Hi Paula,

I usually prefer DMSO for FDA stock solutions because it can be stored in normal reaction tubes in the freezer. For acetone stocks I prefer small glass vessel with a screw lid. Actually, it doesn't matter which solvent you're using, I never observed difference in staining pattern.

Gerhard

Hi Gerhard,

Thank you very much again! You are rigth about glass vessel to keep in stock the acetone dilutions, good point.

Regards

Paula