I have performed all trend protocol to my samples, however my results have very low precisions. If anyone knows and uses any method to measure ACE inhibitor activity , what is the key point in the protocol?
Dear Özlem Üstün-Aytekin,
I believe there are a few methods that you may use.
These are the following references that might be helpful:
1.http://www.ncbi.nlm.nih.gov/pubmed/15708660
2.http://www.dojindo.com/store/p/197-ACE-Kit-WST.html
Best regards,
Wisit
Look in this work, if you have any questions you can contact me.
http://dvr.sagepub.com/content/10/6/498.long
Dear Diego Castro Musial and Wisit Cheungpasitporn,
Thank you very much for your answers.
Dear colleague to all my greetings, I am Surgeon Cardiovascular and promise to ask their scientific concern to researchers from my institution and I will keep you abreast
You can measure plasma level of SDKP.
High Plasma Level of N-Acetyl-Seryl-Aspartyl-Lysyl-Proline
A New Marker of Chronic Angiotensin-Converting Enzyme Inhibition
Michel Azizi, Eric Ezan, Laurence Nicolet, Jean-Marc Grognet, Joël Ménard
Hypertension.1997; 30: 1015-1019
You might be interested to know that a peptide called Ac-SDKP is metabolised exclusively by ACE. Thus, ACE inhibitors will induce an increase of Ac-SDKP in Urine.
this is a very good marker for ACE inhibition (and ACEi compliance).
We do have an ELISA kit to measure Ac-SDKP concentration.
See Jose Alfie Answer.
http://bioreagent.bertinpharma.com/pr15782/acsdkp-eia-kit
Article Acute angiotensin-converting enzyme inhibition increases the...