The main driving force for aggregation is hydrogen bonding and weak electrostatic interaction
I have a problem ligating a small insert with larger vector. The ligation sites are XhoI and AgeI. After the double digestion I excised band for I and V and extracted DNA by glass milk, purified...
11 December 2013 7,759 4 View
I just wanna know if there is any way to predict its biological function such a based on library of functional oligonucleotide known.
31 December 2012 6,494 3 View
I have a problem in the separation of small DNA by Agarose Gel Electrophoresis (though I optimized with different concentrations of gel). Separation was very poor and always very blurry, smear...
31 December 2012 3,864 19 View
We knew Tg values of two polymers before coupling. If they are chemically coupled together, is there any theory or available software to predict the resulting Tg? We of course measured it by DSC,...
31 December 2012 3,952 23 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...
27 July 2024 5,748 0 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...
23 July 2024 9,938 1 View
Hi, I am a PhD student working at Jagiellonian University, Faculty of Chemistry. My work is related to the development of new analytical methods for the quantification of short-chain fatty acids...
22 July 2024 5,492 6 View
I know that films and other low-thickness laminated products are produced using biaxial orientation, but is there something similar for sheets with a thickness of around 6 mm? Or are they...
21 July 2024 1,559 1 View
Dear Researchers, My question is associated with the "MDCI" method in Orca. Please let me clarify my question using a simple example: Suppose we are going to perform CBS extrapolation using "!...
21 July 2024 1,632 0 View
We're studying the stability and fusion behavior of multiple PVC compounds in a Brabender. After a few trials, we set the temperature at 195 °C and the rotor speed at 70 rpm. Samples differ in...
12 July 2024 2,768 1 View
what are the main points
10 July 2024 9,236 5 View