Kien Xuan Ngo

5 Questions 19 Answers 0 Followers

Questions related from Kien Xuan Ngo

Any good suggestions for ligating big plasmid (11 kb) with small insert (250 b)?

I have a problem ligating a small insert with larger vector. The ligation sites are XhoI and AgeI. After the double digestion I excised band for I and V and extracted DNA by glass milk, purified...

12 December 2013 7,743 4 View

Any suggestion for running electrophoresis with small ssDNA, RNA (12mers)?

I have a problem in the separation of small DNA by Agarose Gel Electrophoresis (though I optimized with different concentrations of gel). Separation was very poor and always very blurry, smear...

01 January 2013 3,840 19 View

If I have a sequence of ssDNA oligonucleotide such a 5'CTC TCT CTC TTT3' how do I get its possible biological information?

I just wanna know if there is any way to predict its biological function such a based on library of functional oligonucleotide known.

01 January 2013 6,466 3 View

How to predict the changes of Tg of a certain polymer after grafting with another polymer?

We knew Tg values of two polymers before coupling. If they are chemically coupled together, is there any theory or available software to predict the resulting Tg? We of course measured it by DSC,...

01 January 2013 3,929 23 View

Does anyone know how to disagregate the raspberry like micelles into individual micelles in water?

The main driving force for aggregation is hydrogen bonding and weak electrostatic interaction

01 January 2013 3,082 0 View