We make several transfection reagents for primary cells, and they differ based on the nature of the cells (i.e. cancerous or non-cancerous). For primary neurons, effective lipofection approaches can get to 70% efficiency in cancerous cells (https://altogen.com/product/neuro-2a-transfection-reagent-neuroblastoma-cells-ccl131/) and likely lower efficiencies using generic reagents in non-cancerous cells (https://altogen.com/product/altofect-transfection-reagent/). If your cells are not dividing quickly, transfection efficiencies will be much lower than that for established and fast-proliferating cell lines.

Hi, perhaps you can try GenMute (http://signagen.com/index.php?main_page=product_info&products_id=757).

Cheers

I used to employ HyPerfect (Quiagen 1029975) and it works quite well, and the protocol is very easy!

Look at Mirus. They have several transfection reagents and one specific for neural type cells. https://www.mirusbio.com/products/transfection/transit-neural-transfection-reagent

I used DharmaFect.

http://www.thermoscientificbio.com/uploadedFiles/Resources/basic-dharmafect-protocol.pdf

Thank so much all of you! I really appreciate your information. Now I'm checking the prices of the products mentioned.

We make several transfection reagents for primary cells, and they differ based on the nature of the cells (i.e. cancerous or non-cancerous). For primary neurons, effective lipofection approaches can get to 70% efficiency in cancerous cells (https://altogen.com/product/neuro-2a-transfection-reagent-neuroblastoma-cells-ccl131/) and likely lower efficiencies using generic reagents in non-cancerous cells (https://altogen.com/product/altofect-transfection-reagent/). If your cells are not dividing quickly, transfection efficiencies will be much lower than that for established and fast-proliferating cell lines.