This is question seems to be of very general nature, what exactly is your goal / scenario / challenge?
Do you have a set of segmented images and you want to add your payload to this segmented areas? Do you need information on how to do it? Or do you need information about prior work in this field?
Here are some of the papers which used segmentation for steganography. Hope it will help you to solve your doubts. Please provide details of the project so that i can help you more.