Does anyone know about methods of histones isolation?

More Emilia Stec-Martyna's questions See All

ECG and children with autism ?

Does anyone have experience with collecting ECG data on children with autism? How was your experience? I'm interested in the tolerance levels for kids to have wires attached to their chests. What...

02 February 2021 5,850 3 View

What are the "units" in RNA seq data processed by RSEM?

Hello! I've been using gene expression data from the TCGA, where the RNAseq is processed using RSEM. I did a few graphs such as boxplots showing the expression of different genes in normal vs...

14 October 2020 2,721 2 View

Which is best way to purify my His-tagged protein after eluting from a Ni NTA column in denaturing conditions (urea 8M)?

I have cloned two versions of my protein of interest to produce antibodies in mice. It is not soluble in native conditions, so I am trying to purify it in denaturing conditions with urea 8M. Now,...

18 February 2020 7,305 3 View

Multi-layer wells "robbed" of pumping rates?

By the way, I send attached the workflow I've performed. It is strange that in the Link to parameters window, in the Topology processing, instead of "no topology processing", now what I get is "no...

17 February 2020 8,662 3 View

How to reject authorship of some papers?

You added in my published paper list some article that don't belong to me. The author has the same family name as me, but is being active in a total different area of research. Thank you in advance

24 January 2019 5,795 3 View

Is there an easy way to count the diatoms in a rock sample in a short amount of time?

Hello! I have a bit of a struggle with my diatomites samples. I have tried all the methods to break the diatoms from the rock but I got a negative results (they are highly silicified) and I want...

04 September 2018 6,353 3 View

Any recommended (free) software to rectify and post-process coastal oblique images?

We have a fixed time-lapse camera (RGB) located on a hill oriented towards a sandy coastal area. Images are taken every 30 min. By using a set of ground control points (GPS) we aim to rectify and...

24 October 2017 4,632 5 View

Cancer neoantigens and inflammation

Hi, I am new to the field of immunology. I am trying to understand the different sources of cancer neoantigens and they can be utilized for immunotherapies. In one of the books, I read that...

17 June 2017 8,982 3 View

Is it possible to use Monte Carlo simulation to get prediction and confidence bands for non-linear regression?

I've found simple explanation how to use monte carlo simulations to evaluate parameter uncertainties for non-linear regression in following publications: Lambert, Ronald J. W., Ioannis...

05 February 2017 3,442 2 View

Does Pfu Taq polymerase generate 5'P end?

Does the PCR product amplified using pfu polymerase have 5' phosphate groups to use for blunt cloning?

22 June 2016 1,058 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View