Does anyone know about methods of histones isolation?

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Can I use DNase I and RNase R in the same moment?

Hi, I want to obtain circular RNA and lariat. I need treat my samples with DNase I and RNase R. Do you know, I can do this in the same time? And maybe do you have any protocols for simultaneous...

02 June 2024 9,544 0 View

What are the possible effects of DTT or β-mercaptoethanol, on gDNA amplification?

I used the Intact Genomics FastAmp® Plant Direct PCR kit and when seeding the gel with the PCR product there was a lot of DTT smell. After running the gel, partially degraded dna was observed,...

10 April 2024 2,150 2 View

How can we accurately determine the lactate threshold when there is a delay in the appearance of lactate in the blood?

I am well aware of the methodology for determining lactate threshold; that is not my question. My doubts concern the physiology of lactate transportation to the blood. Once lactate is formed in...

26 February 2024 4,579 0 View

Is there a way to measure firefly and renilla luciferase luminescence in organoids grown in matrigel?

Hi, I am interested in performing a reporter assay in organoids grown in matrigel. Is there a commercially available kit that would allow me to read both firefly and renilla luciferase...

06 February 2024 6,029 0 View

It's possible to know the weights that the package deaR (in R program) assigns to each input?

Hi! I want to ask if is there any way to know the weights that the package assigns to each input to make the analysis. If is there any way that would be awesome to my research of forest Regards!

06 November 2023 4,316 0 View

Hi! Does anyone know of references using strip transect data for habitat selection analysis, or more specifically resource selection function (RSF)??

I have GPS locations of animals in which I will use for a resource function analysis. However, this data follows the transects from aerial transect counts and are aligned on lines. I therefore...

19 October 2023 8,438 1 View

Why do I get an arc when I electroporate Agrobacterium?

I prepared electrocompetents of the AGL1 strain following the "water" protocol: the bacterial culture was resuspended in water, subjected to two washes in water, one in 10% glycerol, and finally...

17 October 2023 4,770 3 View

What kind of test tubes do you use to prepare bacterial suspensions in biomerieux densitometers?

Hi, We are currently using densitometers from biomerieux to prepare bacterial suspensions. We are wondering if there are any tubes with sterile NaCl solution available on the market other than...

25 September 2023 9,673 4 View

What R program or python program do you recommend for analyzing RNA for secondary structure and finding sequential-structural motifs?

Hi! I'm using an R program and I have a large table where I have UTR sequences. I would like to parse these sequences from within R (or python) for secondary structure prediction and finding...

08 March 2023 1,835 2 View

What is recommended set-up for laboratorty steam distillation at a reduced pressure?

Most textbooks provide details about either steam or vacuum distillation. I am looking for a description of the apparatus for distilling with saturated steam at reduced pressure. Do you have any...

13 February 2023 9,276 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How can major oxides determined by ICP-MS/ES be normalized to 100% volatile free? How does this connect with LOI?

I am analyzing cherts from different sources found within one specific geologic formation using ICP-MS/ES. My committee was asking this question about my analysis. I was not sure how to answer this

05 August 2024 7,902 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,084 6 View