I am trying to align sequences using BOWTIE2 which is included in a RNA sequence analysis workflow. The approximate size of the fastq data file is 2 GB. The reference genome file is in fa format and the approximate size is 7 MB. I would like to know the expected size of the sam file. Is there a way to calculate it? (I am not working with linux OS. I am working with a virtual box (with linux) inside windows host?