Does anyone know a protocol for low-scale antibody-HRP conjugation?

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Michael G. Weller

Could you describe your experiment in more detail? Most researchers do not label their primary antibodies directly but use a commercial secondary antibody-HRP conjugate. Another option is to biotinylate your antibody and use a commercial streptavidin-HRP conjugate.

Falk Fish

Stjepan's protocol is fine.

If you wish to learn more about conjugation and enzyme immunoassay get this book (used): https://amzn.to/3hxzIBL

Worth every penny - the Bible of ELISA