Does anyone know a perfect method for measurement of ROS in Arabidopsis leaf?

Ramin Bahmani @Ramin_Bahmani

04 April 2014 1 7K Report

I want to measure the accumulation of ROS (hydroxyl, superoxide anion, etc...) in Arabidopsis based on NBT staining.

Any help is appreciated.

Elisa Graña

Dear Ramin, you can find valuable information in the following link:

https://www.researchgate.net/post/How_to_detect_ROS_production_in_Arabidopsis_tissues2

Good luck!

Badges
Science topic

Similar topics
Agricultural Science

More Ramin Bahmani's questions See All

Can anyone help with a smear of the digestion products on agarose gel?

06 July 2014 4,667 5 View

Can anyone suggest a vacuolar targeting sequence?

05 June 2014 6,312 5 View

How can I measure the nitric oxide level in plant tissue?

03 April 2014 5,216 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Aspen Plus Simulation?

Greetings When one is to perform Simulation or modelling of Pyrolysis of Biomass Waste materials using Aspen Plus software, and one has both the macromolecules breakdown (Cellulose, Lignin and...

06 August 2024 5,023 1 View

Measuring the Intelligence of a Species?

Larger brains, which typically contain more neurons, store and transfer more information (Tehovnik and Chen 2015), but the precise relationship between number of neurons and information has yet to...

05 August 2024 1,238 2 View

How does one derive the standard deviation of a scale?

Dear all, I am working on analyzing data from a survey on student satisfaction. The survey contains items with a 7-point Likert response format that produce 12 scales related to different areas...

05 August 2024 2,141 4 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Any idea about 'International Research Journal of commerce , arts and science? Is it a UGC listed journal?

Any idea about 'International Research Journal of commerce , arts and science? Is it a UGC listed journal? Kindly advice

04 August 2024 7,367 3 View

Are authors sowing for scientific Journals to be reaping the benefits? We are charged for publication but we offer free peer review services, why?

As authors and academic writers, we usually prepare our manuscript using our own resources. We submit these manuscripts to scientific Journals for peer review and publication sometimes at a fee....

03 August 2024 7,304 2 View

Why aren't most metal oxides insoluble in water?

Why BaO (s) + H2O(l) ---> Ba 2+(aq) + 2 OH - (aq) if Oxygen loves owning all the electrons, why would it bind to hydrogen which competes with Oxygen for electron ownership more so than...

03 August 2024 5,392 1 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View