What is the UV-vis spectroscopy and HPLC UV detector?

I did spectrum scan to levothyroxine sodium which show highest absorption at wave length=230nm which the absorption =0.136 and it's absorption at 225nm =0.047 but when Injecting it on HPLC...

09 February 2021 1,631 3 View

StreamPro/Workhorse ADCP underwater tomography accuracy?

Dear colleagues, I am trying to measure low inflow velocities (I expect less than 0.015 m/s) in a stream using ADCP. Comparing to another approach, I found that both approaches are in a good...

16 September 2020 8,774 4 View

Applying the bias correction factor outside the evaluation period ?

I would like to ask an important question. If I have a climatology observed data of potential evapotranspiration for the period 1968-1997 and correct the CRU during this period and there is no...

10 February 2020 4,086 2 View

How to model VLC V2V communication system in matlab?

I want to simulate what will be BER vs SNR if i sent a stream of bits through VLC in outdoor system; V2X or V2V. any help??

09 February 2020 6,478 4 View

How to construct long term time series of temperature using CRU?

If I have 10 years of mean temperature (2001-2010) and evaluate its performance against station observations and finally calculate the climatological bias correction for each month. Can I use...

23 March 2019 4,409 3 View

How to remoe imaginary part of Zin in hfss?

i am working on 6 MHz antenna and i want to remove the imaginary part in the input impedance. i used Lumped RLC bounday condition but no sol. any help?

11 September 2018 2,320 3 View

How important is students' feedback in making changes/adjustment in the curriculum?

What strategies do you have at your school to receive feedback from students? What methods to use to receive feedback from students? Do you use formal and non-formal approaches? How do you handle...

08 June 2018 7,252 14 View

No title

Problem-Based Learning (PBL) has been used in medical and health-related education for over 40 years. Team-based Learning (TBL) has been tried in some schools over the last 10-15 years, one would...

26 October 2017 5,114 4 View

How to apply Pfafstetter Coding method on ArcMap?

07 May 2016 2,109 2 View

How much data is available on Kyasanur Forest Disease (KFD) in India?

Kyasanur Forest disease (KFD) is caused by Kyasanur Forest disease virus (KFDV), a member of the virus family Flaviviridae.

14 August 2014 9,072 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to prepare electrolyte of NaClO4 salt for Na-ion battery? Any problem with this salt?

Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...

21 February 2021 8,132 1 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View