I Read several papers recently and they all used the tRNA in the hybridization buffer. Is there someone who can tell me the reason?
Hi Xiaohui,
The tRNA is there as a nonspecific blocker and carrier to protect the probe and target. Since these are very unlikely to bind specifically to any gene of interest and were readily available and cheap from the first development of hybridization techniques this has become a standard component of nearly all such buffers.
Hi Xiaohui,
The tRNA is there as a nonspecific blocker and carrier to protect the probe and target. Since these are very unlikely to bind specifically to any gene of interest and were readily available and cheap from the first development of hybridization techniques this has become a standard component of nearly all such buffers.
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