Looking for a reliable recipe for making a native gel. Tried a couple I found online, but they haven't yielded polymerization.
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
"A high-fat diet promotes cancer progression by inducing gut microbiota–mediated leucine production and PMN-MDSC differentiation"
13 May 2024 1,321 1 View
Or, really, one of the many other organometallic catalysts that can get this done. From what I've seen, there is no clear answer yet. Perhaps something to do with d-block electron activity but...
28 April 2024 6,666 1 View
We would like to manage in an efficient way our newly conceived flow cytometry and and would like to find a good way to manage our bookings Please Help
21 April 2024 845 1 View
Hi All, After I removed and so the probe the Magpix started reading bead counts of 0 on a test plate with beads only (no sample - no incubation steps), with nothing showing up on the...
27 February 2024 5,898 0 View
can anyone suggest a free 3D simulation software for doing simulation study of organic solar cells?
31 October 2023 1,560 3 View
can anyone suggest free softwares for DFT calculations?
19 October 2023 6,144 5 View
is it necessary to starve cells while using a dual luciferase assay to detect expression on TGF-b cell signaling? I will perform an experiment and I would like to know the pros and cons of...
01 October 2023 6,463 2 View
Hello fellow scientists! I'm currently trying to construct a vector for agrobacterium-mediated transformation into rice. Our construct aims to overexpress two proteins (protein A and protein B),...
14 September 2023 6,194 3 View
Hello Dear all! I would like to know if it is possible to have a stable cell line with a response element attached to a luciferase signal, also, Could it be possible to have in the same stable...
10 September 2023 2,670 2 View
How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...
10 August 2024 942 3 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it
06 August 2024 947 1 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks
02 August 2024 5,748 4 View
Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...
01 August 2024 4,606 7 View
I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...
31 July 2024 9,697 0 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View