Would anyone be willing to point me to or share with me a quantitative extraction and HPLC method for the analysis of carotenoids from animal tissues? Thank you for your help. Best wishes.
I collaborated recently with Kevin McGraw to extract and quantify carotenoids using the following methods and it worked out pretty well. Hope this helps.
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Contact Jeremy Heath . Half of his dissertation dealt with extracting these from gall midge tissues and he ran them in my lab by HPLC. He would have all of the details, however.