How can I extend pot life of PUR with additive?

I try to find the way to get a extend of pot life of PUR (polyurethane rigid). I use Polyol + Isocyanate (MDI) and my objetive is increase te time to work the the mixing. I use oil castor but I...

22 June 2020 9,202 11 View

Direct SDS (Laemmli) Lysate for Western blotting?

Dear all, In the past I use a siimple and fast method to prepare my tissue or cell lysates. Usually snap freeze the tissue and after direct dissociation (sonication/ultrathurax) and boiling,...

29 August 2019 1,187 0 View

How to obtain latent heat of solid-state phase change?

We are studying a heat transfer problem of maraging steel 300. There may be a phase change (from martensite to austenite, at ~600 deg C), and I wonder if the heat associated with that phase change...

24 April 2019 9,237 3 View

Ethic Hackers & The 'Deep Web'

25 July 2017 5,833 1 View

How to get a 'risk of bias summary' to display 'unclear' risks in RevMan 5.3?

This is a practical question about the risk of bias summary in RevMan 5.3: When I make a RoB summary, it only shows the '+' and '-'. Where the RoB is unclear, the box is empty, whereas it should...

21 April 2017 7,196 19 View

How to rate the quality of evidence (GRADE-approach) for outcomes with a non-significant result?

I am working on a systematic review and meta-analysis of RCTs and I am wondering if there is a different approach for rating the quality of the evidence if the difference is statistically...

04 December 2016 7,845 2 View

Can you measure brain cholesterol content without mass spec?

11 October 2016 6,619 2 View

Can somebody help me with some fibronectin coating issues?

03 March 2015 7,678 6 View

Is anyone else doing comparisons of granular and prilled urea on pasture or crops, so I can compare findings?

Prilled urea (0.8-3.8 mm) has become as easy to get as granular urea in the last 1o years. It spreads very well, and weight-for-weight supplies 10 times as many particles as granular urea. This is...

11 December 2014 6,419 2 View

Has anyone tried computationally converting RNAseq data to what it would be if that same RNA had been subjected to an expression chip?

Converting RNAseq data into Exon 1.0 or U133 Chip Data?

05 August 2012 1,199 12 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View

Antibody amino acid sequence database to simulation?

Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...

18 February 2021 371 4 View