with a U bundled heat exchanger or any other heat exchange device
I believe the reason nobody has anwered this question is because their is not enough detail about the material yu are trying to cool, the cooling media and the heat exchanger geometry and design features to give you an adequate answer
I am looking for documents relating to the permission of the US Office of Military Government in post-war Germany in 1946 to allow a single person to found a publishing house / media firm for...
14 June 2023 7,754 2 View
Hello, I am not very familiar with hyperspectral imaging and agriculture, but I am very interested in machine learning models used from data generated by hyperspectral cameras flying over farming...
29 August 2021 1,296 2 View
Hello, I am just starting to research about applications of hyperspectral imaging in agriculture. I am looking for university project, start-ups etc. which gather large hyperspectral data over...
18 August 2021 2,281 3 View
Hello, do you know a common ph Value for the first downstream step in IgG mAb facturing? I would guess around 7 but not sure.
26 September 2019 9,542 0 View
Hello, am I right that in Inclusion body processes the chemicals mentioned above are mainly used for cell disruption and removal of cell debris? Which of them do I use for solubilization of the...
25 September 2019 8,476 4 View
Helllo, if I want to set up a process which wants to manufacture molecular Antibodies and I want to separte them from cell debris, DNA etc. as a first separation step, are there any general...
20 September 2019 9,195 3 View
Hello, what would be characteristical structural/chemical/modificational differences between human and mice antibodies for all or specifif Ig classes? Best, Dennis
12 September 2019 1,907 3 View
08 September 2019 8,970 3 View
Hello, what is the minimal particle size a typical modern depth filter can remove in in a first or second (after centrifugation) step within a downstream process to clarify cell culture broth?...
08 September 2019 6,674 2 View
08 September 2019 9,302 3 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...
10 August 2024 6,493 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
06 August 2024 4,611 0 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View