02 February 2015 2 9K Report

I have tried to purify some proteins from transient transfected mammlian cells, but I have encountered some problems, such as the lipid removal. I found it is difficult to get clear supernant without lipid, I tried ultracentrifuge, Ammonium sulphate precipitation.

The cell line I used is 293T.

Do you have some ideas to remove the lipid from the cell lysate?

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