How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

What is the best way to isolate multiple Salmonella serovars from a single sample?

I am trying to isolate multiple Salmonella serovars from a single food sample. I started by incubating the sample in a non-selective BPW enrichment and from there used multiple selective...

19 January 2021 8,365 4 View

No title

Hi there! I'm very new to r and trying to run a multi-level meta analysis using pre-calculated effect sizes. I've attached the datafile below. The script I used as a first step to fit the model...

12 January 2021 8,214 2 View

Non parametric alternatives to one-way ANOVA for 4 groups?

Hi, Currently assessing NDVI data (vegetation index, continuous data) against land type (4 groups, categorical variables). My data is not normal, or has equal variance (transforming hasn't...

14 December 2020 7,796 3 View

How to remove the jacketing from the midsection of an optical fibre?

I have a HCS fibre with a 200 micron diameter. I want to remove the jacketing in sections along its length so I can replace the cladding underneath and create an evanescent field sensor. Obviously...

13 December 2020 3,068 2 View

the lack of promotion bringing dissatisfaction on the employees?

Write a report with literature review and recommendations for The relationship between lack of promotion and dissatisfied employees to apply your management role to resolve for employees.

21 October 2020 6,698 2 View

How do you extract DNA from buccal swabs submerged in 70% ethanol using Qiagen DNeasy Blood and Tissue Kit?

Hi, I collected buccal swabs (cotton and foam) from nestlings and adult birds and stored the swabs in 70% ethanol. There are few different protocols on the web but I am wondering if anyone has a...

11 October 2020 6,103 3 View

Please help: R problems with forest plots ??

Hello, everyone! I am having trouble running my syntax for my forest plot. Below is the syntax I have used previously, with the changed data frame for my current analysis. For whatever reason, the...

27 June 2020 641 1 View

Does anyone know of a parent-report measure of child loneliness suitable for kids between 6 and 12?

This will be used in a study of children's well-being (we will only have access to parents).

23 March 2020 8,355 3 View

How to calculate substrate consumption rate?

Hello lovely people, It is my first question here, so I hope you can help me.. For an experiment of my thesis I provided pure cultures with different concentrations of lactate an monitored them...

26 February 2020 1,114 3 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Multi effect distillation modeling in EES?

Dear colleagues If anyone has a model of multi-effect distillation in EES or MATLAB software, please send it to me.

24 February 2021 7,282 1 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Agarose gel purification pre MinION sequencing?

Dear all, we performed an agarose gel purification with MinElute kit (QIAGEN) to extract bands between 3500 bp and 6000 bp for further sequencing with MinION. After the purification process,...

16 February 2021 5,178 1 View