Books on Monte Carlo methods?

Hi everyone. I took a basic course on Markov Chains, and know a little about Monte Carlo Stimulations and Methods. But I never got to the part of spreadsheets. If anyone can help direct me to a...

13 February 2021 5,261 3 View

How do we decide dimensions of antenna?

For standard shape (Rectangular, circular) microstrip patch antenna, standard formulas are defined for calculating dimension but for other design how do we decide shape and its dimensions? Does...

22 December 2020 5,535 3 View

How to compare survey results over time in SPSS?

I am completing a quality improvement project where I tested participants PHQ9, SDS, and WHO5 weekly over eight weeks. There are two groups (intervention & TAU). I am SPSS-phobic and not very good...

02 October 2020 7,070 3 View

What are suggest articles or books relating to Game Theory?

I am looking for a good couple of reads on game theory, maybe with an historical context. Anything like random walks, or Marvok Process is fine or even, Colonel Blotto, and Prisoners Dilemma. I...

30 July 2020 710 3 View

How can we differentiate film studies academic research paper from usual literature academic paper ?

Research paper with novel and film adaptation>

25 July 2020 1,158 3 View

Action potentials contaminating my E/I ratio?

I am measuring the effect of a drug on E/I ratio in lateral habenula neurons but I am running into the issue of generating action potentials in the excitatory components. I am using whole-cell...

10 October 2019 586 3 View

How to do the Dementia Knowledge Assessment Scale using SPSS?

I have done half of the analysis and have reached the fifth step where we do a fraction of the total score. so after that i am clueless on what the next step is, also a syntax of the scale is also...

27 September 2019 2,547 1 View

Can we do multiple paired sample t-tests to test for simple effects in a 3x3 mixed anova?

I am computing a 3x3 mixed anova with time (day0, day1, day2) as the within subjects factor and stress-eating response (less than, more than, normal) as the between subject factor. Both the main...

07 April 2019 1,522 2 View

Recommendations on how to store hiPSC cardiomyocytes before Qiagen RNA isolation?

I will be isolating total RNA from hiPSC cardiomyocytes using a Qiagen kit. Would it be better to freeze the cells straight away, then thaw and lyse, or to add lysis buffer to the cells before...

05 February 2017 6,848 3 View

Recommendations on tools to check primer alignment to gene of interest CDS?

16 January 2017 5,690 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Which reagent is best among 'Dimethylglyoxime" and "Dithiothreitol" for spectroscopic determination of Nickel ?

What I think is Dithiothreitol. As it is water-soluble and does not form a precipitate. But, the thing is it contains sulphur which is quite hazardous to aquatic animals.

01 March 2021 8,374 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View