Does anyone have experience with markers for murine urogenital sinus - in situ hybridization?

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Hlw, I am trying to build a model a single pile in 3 layers soil in Flac-3D. Could you please help me about the Flac-3D code?

I am facing difficulties to insert the pile in soil layer and define the pile exactly through Flac-3D code.

22 June 2024 8,334 1 View

How to reduce notches in input AC voltage for Totempole Power factor Correction circuit?

Respected readers, I am working with a totem pole PFC circuit. In an experimental study, I face a problem of high-frequency notches in source voltage (Yellow one). I found an explanation behind...

14 May 2024 6,692 6 View

How Wind response spectrum like Davenport implemented on a structure Numerically?

Davenport wind spectrum can calculated through software, but I couldn't understand how it is implemented on a structure through software application. if anybody could enlighten me, it would be a...

05 May 2024 9,728 3 View

How to detect the polymorphism of a material with monolayer thickness?

We have grown boron nitride with a monolayer thickness. XRD usually is used to understand the polymorphism of any material. However, due to the minimum thickness of the sample, it won't work for...

15 April 2024 5,431 1 View

What is the proper procedure of making uniform hydrophobic chrome coated glass wafer surface?

I have deposited chrome on the glass wafer. Then I applied a dielectric layer of SU-8 over it. After that to make the surface hydrophobic, I applied Teflon by using spin coating. However, I found...

12 April 2024 7,009 3 View

Is it good to have antibiotic resistance property for probiotic?

To prevent intestinal disorders, probiotics can be rendered multidrug-resistant to survive in the presence of co-administered antibiotics, and they may also transmit the antibiotic-resistance gene...

01 April 2024 8,043 2 View

Protocol for short term liquid culturing of AML patient derived Peripheral blood mononuclear cells?

Hi everyone! I want to isolate the PBMCs from AML patients and transduce the cells with my lentivirus to knockdown a protein and use for further experiments. I am unable to find a single widely...

14 March 2024 4,317 0 View

How to plot Raman mapping in Origin from raw data?

How to plot Raman mapping data in origin software?

27 February 2024 9,522 0 View

How wind spectrum formed to find wind buffetingloads on vertical slender structure ?

is wind spectrum consume mean wind and turbulence both ? and how the mean wind will be calculated from measured wind data?

04 January 2024 8,546 2 View

Should a specific gene be targeted for comparison or is whole-genome sequencing advisable for a mosquito species lacking a sequence in the NCBI?

What methodologies are involved in sequencing and aligning the genome of a mosquito species for which the sequence is not available in the NCBI? Additionally, how can one construct a phylogenetic...

19 December 2023 6,721 0 View

Stability of the Solar System: Insights from Einstein’s Equations ?

The stability of the Solar System is a complex subject that blends the classical framework of Newtonian mechanics with the modern insights provided by General Relativity (GR). Understanding this...

07 August 2024 2,569 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

I need the datasets of Microgrid for system identification?

Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks

02 August 2024 5,748 4 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

How to Compress Information Neurally?

Samuel Morse, the inventor of the Morse Code, understood that certain letters in the English language occurred more frequently than others (Gallistel and King 2010). To deal with this, Morse used...

01 August 2024 4,456 2 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

Can I change concentration and brand of antibodies mid-experiment?

I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....

29 July 2024 5,961 2 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View