Does anyone have experience growing 3D organoids from cell lines?

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Salvador Flores-Torres

Hey there, some cell lines might not be able to reorganize into organoids. However, optimized cell culture media may promote reorganization.

I am currently doing some research on this topic and I am working with primary samples which will form organoids.

Iftikhar Ali Khawar

Dear, by culturing cell lines under 3D you shall get spheroids not organoids. To get tumor organoids or tumoroids, cells harvested from the tumor tissues/FNA are needed.