Does anyone have experience depleting mouse B cells with anti-CD20, anti-B220 or anti-CD19 antibodies?

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Rogier M Reijmers

As far is I know, these antibodies will not work. Depending on the research question you have, you could try working with humanized CD19/CD20 transgenic mice (http://www.ncbi.nlm.nih.gov/pubmed/20605905) or use the muMT knock out mouse, that lacks B cells.

Good luck!

Rogier

Marta A Toscano

Thank You Rogier!!

Chao Song

Hi, Marta. I'm recently also trying to deplete murine B cells by injecting antibodies. Have you already finished this experiments? Could you give me any suggestions? I have tried anti-mouse CD19 antibody from Bioxcell, but it turns out to be low efficiency.

Noor Bahadar

Have you people ever tried human CD19 for depletion? I think that works better comparatively.