I want to quantify the amount of GLUT3, GLUT4 and maybe GLUT1 in the HPC of rats using Western Blotting. If anyone has worked specifically with these antibodies and has one that has given consistently good results, I'd really appreciate a product number or whatever you can provide! The number of options is overwhelming.
I have been using Glut1 antibody from Abcam, which works good.
You can try.
Hello,
I would recommend checking a free antibody database at labome.com. For GLUT3 (SLC2A3), Abcam has several rabbit polyclonal antibodies against GLUT3 (both anti-mouse and anti-human) for Western blot: http://www.labome.com/review/gene/human/SLC2A3-antibody.html, (Rocha et al. Mol Hum Reprod 2014). In addition, I can see that Santa Cruz provides one goat polyclonal and one mouse monoclonal anti-GLUT3 antibodies. You can also search there for suitable GLUT4 antibodies: http://www.labome.com/review/gene/human/GLUT4-antibody.html. There are antibodies suitable for Western blot provided by Abcam (also rabbit polyclonal anti-mouse), see Hong et al., Mol Med Rep, 2015. Santa Cruz (made in rabbit, goat or mouse, detecting either human or mouse GLUT4) and EMD Millipore. Regarding GLUT1, there are antibodies available from the same companies http://www.labome.com/review/gene/human/GLUT1-antibody.html. Hopefully, this suggestion is helpful.
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