I try to amplificate CGG repeat expansion in FMR1 gene but i only get to 75 repeats max. I tried several CG-rich amplification kits but nothing worked. Any ideas or experiences? Thank you guys!
Dear members, I am investigating the effects of Artificial Intelligence (AI) on marketing for study purposes. The survey will only take a few minutes, and your responses are anonymous. Thank...
07 April 2024 2,241 3 View
For a industrial application (layout-planning) I am currently trying to globally optimize a discontinuous function f. The objective function is defined on some bounded parameter space in R^N where...
26 February 2020 6,679 5 View
I am unable so far to find any records on changes of annual or decadal sales, production or usage of fungicides based on mercury compounds. Such fungicides were for example Uspulun and Ceresan (by...
14 December 2016 344 3 View
Does anybody have a practical experience with angular or energy filtered of BSE or SE signal in scanning electron microscopy? Would you recommend me any article with applications shown? Thank you
21 January 2015 4,303 2 View
Hello, Can anyone advise me how to eliminate artifact as shown on the picture here: http://prntscr.com/4pso6q I am concerned about vertical stripes. I think it comes from the knife cutting the...
23 September 2014 5,969 3 View
The Graph below shows an example of an amplification curve that shows an overshoot in fluorescence. I am using SYBR Green, BST polymerase, and 6 LAMP primers in the mixture and amplifying at 65...
14 July 2024 1,483 0 View
Hello. In our laboratory, we are currently conducting Loop-Mediated Isothermal Amplification (LAMP) to detect insert DNA in plasmid vectors. When using the LAMP method, we typically use 8 tubes,...
24 June 2024 645 1 View
I have designed primers for HBB Gene and the template is Whole Genome amplification(WGA) products. The primers work in some samples and in some they don't. I measured the OD of both the samples....
23 June 2024 3,361 2 View
I am conducting a LAMP assay, and while the negative control shows no bands as expected, my positive control samples consistently display only a single band instead of the anticipated ladder-like...
18 May 2024 4,853 3 View
I am looking for any research article that demonstrate the effect of different length and Tm of Primers used in LAMP reactions. Does the Bst 2.0 Polymerase have any impact on the Tm of primers?
28 April 2024 5,729 0 View
I had transformed my desired gene along with selectable marker into rice plants and started with gene expression studies. Previously Amplification was observed in both genes but now on repetition,...
14 March 2024 7,841 1 View
Does anyone have any experience with the Nickase Strand Displacement Amplification technique? I am trying to figure out how to design the primers but I need some help :) DOI:...
25 February 2024 9,722 0 View
Dear Friends! The gold-standard for identifying an allele for human leukocyte antigen is SBT or sequence-based typing. If I devise a new PCR test or LAMP test, how many known positive and negative...
16 February 2024 7,952 1 View
Hello, I'm currently in need of performing Loop-Mediated Isothermal Amplification (LAMP). There are modifications in both the Bst DNA polymerases from NEB and ThermoFisher. Has anyone had...
31 January 2024 2,534 1 View
Hello everyone I need your help with a problem I can't seem to solven : I'm planning to do some sequencing of freshwater algae. So I referred to the primer pair made by Stoeck et al. 2010 and...
23 January 2024 5,970 4 View