I am trying to get single, good shape, big crystal for neutron diffraction. After tried several methods, I successfully got single crystal by microseeding. But crystal is still small which is not enough size for neutron diffraction. Is it effective way to get big crystal if I add additional protein sample in to the crystal well after appeared the single, small crystal in to the well? If any one have an idea please give me some suggestions.
What is the size of the crystal you have grown? On the Laue diffractometer Vivaldi at ILL one can measure a single crystal of size 1 mm^3.
You can however synchrotron X-ray diffraction on a much smaller size crystal at the ESRF. There is no need to do neutron diffraction unless you are keen on proton positions of H atoms which can be better determined by neutron diffraction.
Even smaller crystals can be measured on XFEL at Standford or soon at DESY in Hamburg.
Thanks a lot for your answer.Actually we want to determine the proton positions of H atoms.For this reason, we will try to do neutron diffraction.Already we got X-ray diffraction image at very high resolution using our present crystal.But this crystal size (length of crystal 0.3 mm, width is less than 0.05 mm) is not enough to do neutron diffraction.Now we are trying several methods to obtain big,good shape crystal.
If you get a bigger crystal then you can write up an ILL proposal on LADI.
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