Ciao Vanni!

you can talk to people at the CRO, Centro di Riferimento Oncologico di Aviano, they work on Bouin fixed tissues. Check this paper for some names and addresses: http://www.ncbi.nlm.nih.gov/pubmed/22648804

You can look at the papers from Valli de Re here on researchgate: https://www.researchgate.net/profile/Valli_De_Re

You can also contact me directly for e-mail addresses :-)

Ciao Filippa,

thank you for answer.. Sometimes we have the solution near us but we don't know!!! All the best ):::

Bouin's fixative if a great quality fixative able to preserve cell structures and the only interference refers to the picric acid coloration. Remove it with several washes of 70% ethanol and proceed with your normal IHC protocol.