Hey guys, I'm looking for an efficient protocol to transfect patient-derived tumor cells with a high rate of transient transfection?

As you know it's quite tricky to get primary cells transfected at a relatively high rate!

31 January 2021 3,580 4 View

What would be the best option to dock a number of peptides to a protein?

I have been using PyRx to dock peptides to a protein. Initially the numbers were small so it was okay. But now I have to dock over 500 peptides and doing so manually would take forever. My...

10 January 2021 3,330 4 View

What is the effect of drought and silicon stress on soil pH and EC?

What is the effect of drought and silicon stress on soil pH and EC?

15 November 2020 3,971 5 View

How do I get the number of water molecules in a carbon nanotube?

12 September 2020 886 6 View

How can I access the Seqscape software 2.7 ?

Hi, I need Seqscape software 2.7 to analyze DNA sequences because this version supports 3500 sequencers. Please help me how can I access this version of the software? Thanks

17 August 2020 4,693 3 View

Is it possible to use an accumulator as the main supply of oil pressure instead of the pump in a power steering system?

it means the oil pump to be sometimes able to turn off during the driving cycle, and if it is possible, how efficient would it be? (more particularly in heavy vehicles like a bus).

03 April 2020 6,765 1 View

MiRNeasy Serum/Plasma kit abnormally low OD?

We have purified RNA samples using miRNeasy Serum/ Plasma kit using RNase free solutions but still, we have got low OD of purified samples ( 98 and 56 microgram/mL). which actions should be measured?

21 September 2017 1,067 2 View

The method was applied by gaussian software for calculations of BSSE

24 June 2017 7,758 3 View

What reactions is used in MTP(methanol to propylene) process?

All of the reactions in this process

15 May 2017 8,722 3 View

I have used E-cadherin primers for Realtime but I have not seen any melt curves for my samples. what can I do ?

31 January 2017 5,651 3 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

Can I download BibExcel for MacOs?

Hi everyone, I would like to ask how I can download BibExcel software for MacOs? There are some links which are supported by Windows but I could not find it for Mac. or is there any other software...

01 March 2021 652 1 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View