Does anyone have an empty pET28a vector, or know of a resource where I could procure it from?

last month 0 298 Report

I am looking for empty pET28a vector that'd allow my students to express and ultimately purify a protein of their choice using column chromatography. Seems like Novagen doesn't supply it anymore and Addgene doesn't have it in its repository either. Thank you!

Badges
Science topic

More Kamesh Regmi's questions See All

What does an "Error 111" mean on an AKTA Explorer?

Dear Colleagues, I would be grateful if any of you can advise me on what an "error 111" is on an AKTA Explorer. The manual suggests that the instrument be turned off and turned on. That does not...

13 June 2024 2,961 1 View

Where do I obtain Chenopodium quinoa Willd. (quinoa) seeds (SAMN04338310; QQ74) that were originally used for genome sequencing?

The Chenopodium quinoa Willd. (quinoa) seeds with accession PI614886 (Biosample accession code SAMN04338310; also known as NSL 106399 and QQ74) is what I require and was also used for genome...

10 December 2023 5,680 2 View

What is the difference between sample preparation and sample cleanup?

Is sample cleanup not a part of sample preparation. Are there any examples sample cleanup that are not sample preparation in analytical chemistry?

19 October 2023 5,809 8 View

How can we treat the data if two latent variables have perfect correlation while using structural equation modeling?

05 June 2023 7,962 2 View

How does endoparasite develop inside the body ? Are they internally made or go inside the body from external environment through the means of feeds?

If we create an artificial environment to an animal, so that the feeds/grasses/silage/forage, concentrate, and water whatever we provide are totally parasites and their eggs or larvae free, then...

09 February 2023 4,633 3 View

What dilutions of serum sample should I use in ELISA?

I would like to do an ELISA to measure the levels of a biomarker in mouse serum. The concentration of the standards ranges from 2000pgs/ml to 31.25pgs/ml. I have the kit from R&D systems but...

18 February 2022 10,013 2 View

I have a DEM with an ellipsoidal height value. How can I convert that ellipsoidal height into orthometric height?

Query on conversion tool.

14 February 2022 9,245 3 View

What is the best method to get historical satellite imagery except google earth and earthexplorer?

I am looking for satellite imagery of last 10 years so that i can find out change detection in such place. The possible solution to download the historical solution is by using google earth pro...

14 August 2021 5,775 11 View

About non linear analysis?

hello everyone. i have multiple continuous dependent variables rated on scale along with categorical independent variables such as sex, study etc. while checking for linearity i realised that my...

30 July 2021 9,085 7 View

Can someone help me with result section(apa) for my thesis?

hi i have to write my result section for my thesis but I keep on getting stuck because of formatting and issues. for analysis i have have conducted multivariate glm with sex,studyfaculty as...

10 July 2021 1,094 8 View

How to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field in Techplot?

Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You

02 August 2024 3,615 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Where to download DEM from 2018?

I am currently working on validating land subsidence results obtained using SBAS InSAR. Could anyone suggest reliable sources or repositories where I can download a historical DEM from 2018?...

31 July 2024 9,757 3 View

I need help with cloning a 200 bp insert and a 4,7 Kb pEGFP-N1 vector. How can I do it?

I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...

09 July 2024 4,277 1 View

Cloning restrction digestion needed before sequencing?

Hello, recently i am an issue swith cloning where I am using the subclone method to insert my gene length is 1.5kb and my vector(pMAL-c6T) is 5kb I got the colonies which i screened through the...

04 July 2024 7,977 2 View

Why I am unable to get the expected insert at the cloning site?

I am trying to clone a gRNA (23 bases) into the Crispr cas9 pDirect23A vector by using restriction enzyme cloning. I have performed a complete experiment, starting with plasmid isolation,...

02 July 2024 5,448 2 View

What could be the expression profile of alkaline phosphatase from lactic acid bacteria?

I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...

02 July 2024 5,332 3 View

List Plasmid suitable for Homology cassette in CRISPR-Cas9 protocol for Knock-In in mammalian cell line ?

Hello, I am working on creating a mutant endothelial cell line having Neomycin knock-in present, I have designed my homology arms of 1kb size having neomycin in between them, I would like to...

25 June 2024 1,197 0 View

How can I convert remote sensing (RS) images to vector data automatically?

I am working on a project that involves analyzing remote sensing (RS) images. I need to convert these images into vector data for further analysis. I am looking for automated methods or software...

22 June 2024 6,165 2 View

Asking for a vector, containing Hygromycin B resistance gene?

In my work, Hygromycin B resistance gene is require for screening. I tried to amplify this gene from the vector pIJ941 but failed. I will be thankful, if anyone help me out from this problem by...

19 June 2024 7,822 3 View