What would my sampling technique be?

I am having issues explaining my sampling technique in my methodology. I have utilised a two phase sequential explanatory design collecting and analysing the Quantitative data in the form of an...

01 August 2019 4,049 4 View

MG-63 plasmid transfection using Lipofectamine 2000?

Has anyone got any experience of transfecting plasmids into MG-63 using Lipofectamine 2000? I am currently using it to transfect three GFP-containing plasmids - one of which is 7kb and the other...

06 February 2019 3,663 2 View

What may cause the death of mice after repeated immunization with OVA + alum?

I have tried to induce an allergic response in female Balb/c mice. Mice were immunized i.p. with OVA + alum 3 times (days 0, 10, and 20), and after the 3rd immunization, mice started to die. Does...

04 October 2018 5,171 4 View

Using regepochs function to slice the eeg data collected from Emotiv Epoc+ in EEGlab?

Hi all I am completely new to Matlab and EEG, and I've encountered an issue while I was trying to slice a continuous data with no marker captured using Emotiv Epoc+. I am using Matlab R2017b...

20 March 2018 3,075 1 View

Light wavelength attracting pest natural enemies?

Now I am interesting in if any kind of light wavelength could usefully attract pests natural enemies like lady beetle, predatory bugs, hover fly, lacewing, or parasitoids. Could any one could...

10 November 2017 894 3 View

Has anyone been able to successfully extract RNA from nucleated (avian or reptile) whole blood preserved in RNAlater?

I have Avian blood samples taken on site and stored in RNAlater blood tubes. Where I run into problems in at the Prot K/Lysis step. I end up with a solid, jell-o like blob. I know it has to do...

01 November 2017 8,910 2 View

1000 fold increase compare to control in real time PCR, is that normal?

When I over expression a protein and detect the increase of mRNA of that protein using real time PCR, the result show increase 1000+ fold to mock transfection sample. Is this result common and...

26 December 2016 6,923 5 View

If one variable (moderating) is positively skewed, how do you conduct parametric testing?

* Efforts to transform the variable via log and sqrt were unsuccessful

21 May 2016 761 3 View

Should the moderating variable be correlated with the independent and dependent variable?

21 April 2016 2,994 7 View

What can be the problem for very low or non-detectable expression after lentivirus infection?

16 August 2015 5,980 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View