I have been having problems with my PCRs using VNTR primers to detect wolbachia in tsetse flies.
Schneider et al (2013) Journal of Invertebrate Pathology, 112. S104-S115
How do I go about getting primary data for my research ? I am using an Organisation as my research case study but I want to know how to go about collecting Data do I need to approach the...
29 November 2021 8,364 9 View
Possibly on some techniques or methodologies in analysis of forensic sources of data
25 February 2018 3,279 5 View
In using BLA, what’s likely to be the minimum sample size to used for the result to be acceptable and Reliable? I have been studying soil and leaf tissue nutrients response for a berry crop and...
27 January 2018 1,663 5 View
Assessing bush growth (size/volume) of currants and haskap.
23 July 2017 512 0 View
I have being studying the relationship between soil and plant variables. I have a total of 25 variables in 12 location, I'm a little bit confused on the number of PCs to use that will best explain...
25 June 2017 6,438 4 View
Crop yield and growth parameters have been used to establish leaf tissue nutrient optimum standards, why is soil nutrient concentration levels not a reliable or used to establish nutrient norms...
21 February 2017 2,021 9 View
After treating for missing data with EM algorithm using SPSS, I'm getting such outputs (please see attachment). What should I do? Thank you.
06 February 2015 2,245 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
I need to install this program
25 July 2024 4,756 0 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View
I am preparing to run a lot of samples on a 384 well plate and I wanted to test if any well in my PCR machine is dead so as not to lose any data. I would really appreciate help with how I can do...
24 July 2024 8,172 1 View