Does anyone have a working protocol for FISH for telomere probe of the cells growing on silicone membrane?

04 April 2014 1 1K Report

I successfully did FISH for cells growing on cover slip, but the same protocol is not working for cells growing on silicone membrane. Do I need to change the hybridization time, temp or denaturation time temp due to the silicone membrane?

Sugata Hazra

Thanks!

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