Does anyone have a protocol for isolating exosomes from milk?
Chen et al. BMC Genomics 2014, 15:100
The above reference have the procedure below, just in case, If you don't have access to the article, I have copied and pasted it for you,
Preparation of exosomes from milk
Porcine milk samples were centrifuged first at 2,000 × g for
30 min at 4°C to remove MFGs as well as mammary glandderived
cells. Defatted samples were then subjected to
centrifugations at 4°C for 30 min at 12,000 × g to remove
residual MFGs, casein and other debris. Subsequently, from
the final supernatant (so-called whey or milk serum), the
membrane fraction was prepared by ultracentrifugation at
110,000 × g for 2 h in an SW41T rotor (Beckman Coulter
Instruments, Fullerton, CA) .
I hope this works for you.
Hie my friend..found this article may help you its free on net.
Comparison of Methods for Isolating Exosomes from Bovine Milk
Tetsuya YAMADA1), Yasuo INOSHIMA1)*, Tsukasa MATSUDA2) and Naotaka ISHIGURO1)
1)Laboratory of Food and Environmental Hygiene, Department of Veterinary Medicine, Gifu University, 1–1 Yanagido, Gifu 501–1193,
Japan
2)Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464–8601, Japan
(Received 27 January 2012/Accepted 12 June 2012/Published online in J-STAGE 26 June 2012)
Hello
And see these attachment and link
Good Luck
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3369768/
Thank you guys, the articles are a great help
Total exosome isolation (from other samples) reagent - there is a protocol for milk
Thanks Alex
Preprint Charge Detection Mass Spectrometry Measurements of Exosomes ...
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View