Does anyone have a protocol for isolating exosomes from milk?
Chen et al. BMC Genomics 2014, 15:100
The above reference have the procedure below, just in case, If you don't have access to the article, I have copied and pasted it for you,
Preparation of exosomes from milk
Porcine milk samples were centrifuged first at 2,000 × g for
30 min at 4°C to remove MFGs as well as mammary glandderived
cells. Defatted samples were then subjected to
centrifugations at 4°C for 30 min at 12,000 × g to remove
residual MFGs, casein and other debris. Subsequently, from
the final supernatant (so-called whey or milk serum), the
membrane fraction was prepared by ultracentrifugation at
110,000 × g for 2 h in an SW41T rotor (Beckman Coulter
Instruments, Fullerton, CA) .
I hope this works for you.
Hie my friend..found this article may help you its free on net.
Comparison of Methods for Isolating Exosomes from Bovine Milk
Tetsuya YAMADA1), Yasuo INOSHIMA1)*, Tsukasa MATSUDA2) and Naotaka ISHIGURO1)
1)Laboratory of Food and Environmental Hygiene, Department of Veterinary Medicine, Gifu University, 1–1 Yanagido, Gifu 501–1193,
Japan
2)Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464–8601, Japan
(Received 27 January 2012/Accepted 12 June 2012/Published online in J-STAGE 26 June 2012)
Hello
And see these attachment and link
Good Luck
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3369768/
Thank you guys, the articles are a great help
Total exosome isolation (from other samples) reagent - there is a protocol for milk
Thanks Alex
Preprint Charge Detection Mass Spectrometry Measurements of Exosomes ...
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