We want to punch out hypothalamus from frozen mouse brain. I have been suggested to follow Atlas but I am unsure about which coronal section to start or end. Is there a marker to check if the area we are isolating is actually hypothalamus and something else?
Enrico Marani
Use glass pipettes, art is to elongate and break them not oblique. By warming the glass you can make the diameters you want.
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Manju Tembhre
Hi Robin,
If you only wish to analyze the hypothalamus I suggest to "block" the brain, coronal cut anterior and posterior to the area of interest. You can put the other blocks frozen for further investigation.
Also you can use mouse brain slicer for better section. Pl. access link below;
http://www.zivicinstruments.com/mouse-brain-slicers-matrix-section.html
Manju
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