HPLC-UV or LC-MS method
See the Waters website. Why reinvent the wheel?
http://www.waters.com/waters/library.htm?cid=134635960&lid=1518003&utm_content=sf53992780&utm_medium=spredfast&utm_source=linkedin&utm_campaign=Waters+Corporation&xcid=sfwaters-linkedin-BehindTheScience+tc20Biopharm+v-Large+Mol+Biopharm+p-CBU+Multiple/Other+App+Notes+%26+Posters+(no+reg)-sf53992780&sf53992780=1&locale=101
Waters link seems to be amino acids & not the compounds in the original question?
In that case try here. I am sure they have something;
http://analyteguru.com/?s=nucleotide
You can see some papers relation below
Please find attached paper for the info u seek.
regards,
Madhav
It is possible that Waters have a methodology, they work very similar topics.
this may help http://www.sciencedirect.com/science/article/pii/S0039914011005558
This application note might help..
you can use ion-pair method with C18 column to analyze them. pls check the following article.
Article Identifying Biomarkers of Wharton’s Jelly Mesenchymal Stroma...
good luck
here are two latest papers for LC and LC-MS for quantification of nucleosides
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