Does anyone have a good kit for telomerase activity and telomere lenght for rodent samples?

Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

17 July 2024 2,787 4 View

Hello friends In simulating the drug with the liposome, which side of the liposome membrane is the drug placed on? The top leaf or the other?

Several times by placing a restriction on the upper part of the membrane with the aim of preventing the drug from being added from this side, we tried to keep the drug at the top of the leaf, but...

29 June 2024 9,688 1 View

Can I premix the reagent for restriction digestion and make a mastermix like PCR?

Hi everyone, I am currently trouble shooting my restriction digestion, so far it hasnt given me clear bands when I ran gel. I've been adding all reagents minus template, to make a master mix...

24 June 2024 1,171 3 View

Will this 3C Analysis work or are the fragments too small/close together?

I'm doing a 3C analysis shown in the picture linked and attached below with the Bait fragments labeled and the target fragments that will be tested for interaction with the bait labeled with...

02 June 2024 8,038 1 View

What happens to qPCR if extension step is not included?

I am trying to measure telomere length in human DNA samples through qPCR. In the protocol, they have not included extension step. Can anybody explain how a qPCR can work without an extension step?...

31 May 2024 514 2 View

How KAM value are relates with FCGR?

"How will it affect the material? Is a higher KAM value beneficial for restricting cracks, or is a lower value more effective?"

30 May 2024 9,211 0 View

How to scale up or down a restriction enzyme reaction?

Hi I am trying to set up the reactions with my plasmid, insert and RE. Usually the standard final volume is 20 uL or 50 uL (it depends on the protocol provided by the manufacturer) with 1 ug of...

28 May 2024 1,105 3 View

How to set up a restriction enzyme reaction?

I'm a doctoral student with early experiences with recombinant protein synthesis. Since I still have to start my experiment and I do not yet know the quantities of DNA and plasmid that I will...

19 May 2024 5,171 3 View

How can i use RFLP Method for these SNPs ARID5B rs4948496, DHFR rs1650723?

these SNPs do not have restriction enzymes is it possible to do RFLP

15 May 2024 7,974 1 View

Why is my restriction double digest not working?

I have designed and assembled a 10kb plasmid containing SbfI and NcoI restriction sites flanking a promoter region, which I am attempting to excise and replace with other promoters. When I do a...

09 May 2024 2,553 1 View