How to clone pcDNA vector in SH-SY5Y cell line?

Dear all, How I can clone two genes in pcDNA vector, simultaneously (one of them is target gene with 500 bp length and one of them is GFP). Also, how we can transfect the vector into SH-SY5Y cell...

12 November 2020 6,122 3 View

I am looking for a text book on quantum theory which is suitable for mathematicians. Does anyone have a suggestion (instead of B C Hall's one)?

I mean a book which is written in math texts style. I saw Hall's book but I am looking for a book whuch has more details.

30 October 2020 1,636 6 View

How to simulate MSE walls for dynamic FE analyses?

Hi colleagues, Because of the complexity of modelling geogrid layers and their interfaces which can cause convergence problem in FE analyses, I have seen in practice they model the whole MSE wall...

14 August 2020 4,649 3 View

How I can see contact stress in my analysis result in abaqus ?

I want to report contact stress in my result, is that cpress (contact pressure) ? what is the different between contact stress and contact pressure? !

27 July 2020 3,767 3 View

Is it possible to simulate tip-sample interaction during Scanning Probe Microscopy in Abaqus? and if not which software do you recommend?

I want to simulate this interaction in Abaqus but I heard it may be possible only in Comsol, considering the samples are measured in Nano scale, thanks

01 April 2020 8,313 3 View

Does industrial scale Hydrometallurgical purification of Silicon exist?

There are several papers describing different hydro-metallurgical purification routes of Metallurgical Si to Solar Si through alloying, doping, nano sized grinding,... and finally leaching in...

28 March 2020 2,201 3 View

Why TiO2 powder earnd from sulfate process turns yellow after anealing?

Tio2 powder is earned after sulfate process by solving illmanite in the sulfuric acid and a purity of up 95% of TiO2 is gained . why the color of Tio2 turns yellow after 2h annealing in 900 oC...

07 February 2020 5,769 3 View

How to fine grind material in a narrow band particle size distribution?

Does anybody have ideas to mill Silica powder to micron size particles to a narrow particle size distribution (PSD)? For example grinding with planetary ball mill makes a wide PSD which is not...

19 December 2019 5,431 2 View

Hi all, could you please recommend a good review paper on the scheduling of renewable energy resources (RESs) in microgrids?

I would be very thankful if anyone could recommend some good review papers about the scheduling or economic dispatch of RESs in the microgrids. The assessment of the greenhouse gasses in the...

05 December 2019 6,335 3 View

Diffused dye front on SDS-PAGE, any suggestions on what the problem might be?

I ran these 15% gels for 90 minutes under 200 volts ( gel ran too slow), ladder looked very condense on the top and the dye front was somehow diffused at the bottom of the gel. I am sure about the...

16 April 2019 6,092 5 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View