Does anyone know of a protocol for dissociation of adult mouse brain hypothalamic neurons to be used in acute calcium recordings? We are not interested in doing cell culture, but rather using the cells acutely after dissociation.
Thanks!
Hi,
You can acutely dissociate hypothalamic neurons using papain for 10-15 mins and then wash the cells with PBS with glucose and use them for your calcium recordings.
Good luck!
We have optimized protocol for dissociation of adult hippocampal neurons for Ca2+ recording. Please see attached.
For hypothalamic neurons, the choice of enzyme and final plating medium will be critical. See: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2578824/
Hope this helps. Good Luck!
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