Dear FACS gurus,

Can apoptosis (Annexin V (FITC) + PI (PE)) be co-stained with beta-gal probe on any of the remaining channels (using CantoII in either Red laser 660/20nm APC, APC-R700, AF647/ 780/60nm APC-Cy7, APC-H7, AF 700, Draq-7 or Violet Laser, 450/50nm Pacific Blue, BV450, BV421 510/50nm Amcyan, BV500, BV510) ?

If so which beta-gal reagent do you use please?

Is it advisable to run these 2 assays together at all or best keep separate?

Many thanks,

Ilze