How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Apricot database - where to search?

I work in apricot research and we have a breeding program for abiotic stress tolerance. I collect data from the cultivars i could use in this program individually, but there are several hundred...

01 February 2021 386 3 View

No signal/bands observed on western blot for ERK signaling?

My question is regarding a western blot experiment that I am performing to observe pERK signaling by stimulation with VEGF ligand (VEGF165 and VEGF121) on MDAMB231 cells and SW620 cells (both...

30 January 2021 1,039 3 View

How I Can calculate the resistance of each vessel in vascular system based on inlet pressure condition and outlet flow rate?

I have an ordinary vascular system, where I know the inlet condition of pressure on the system and value of flowrate through each outlet. Based on these two pieces of information I need to...

16 December 2020 1,599 1 View

Cell line that does not express any of the VEGF receptors?

I am looking for VEGFR-negative cell lines, that either show very low or no expression at all of VEGF receptors for cell signalling assays.

01 November 2020 6,046 1 View

Which methods (or modern programms) are used to assess the biodiversity of highest vascular plants?

27 October 2020 240 3 View

What are the genetic/physiological differences between deep water rice cultivars of North-Eastern India from that of South East Asian Countries?

Deepwater rice cultivars found in various regions around the world have their own differences. Please mention how the deepwater rice cultivars of North-Eastern India varies from that of Southeast...

23 October 2020 1,333 3 View

Is there a database indicating the carbon and nitrogen storage capacity of vascular plants (in Europe)?

Hi everyone, I search a database or a review listing the capacity of vascular plants to storage carbon, nitrogen or other important chemical elements. I work actually on a mountain area (Swiss...

19 October 2020 8,596 3 View

Can anyone suggest some potential target proteins for anticancer docking studies?

The plant extract, I have been working on, has a potent anti-tumor effect proven by several in vitro and in vivo studies. Through, the direct effect of the extract on tumor cells is not explained...

11 October 2020 2,935 4 View

Vascular Reactivity Protocol (Organ Bath): How to test endothelial integrity?

I've read several studies analyzing vascular reactivity (mostly aorta, mesenteric and femoral arteries) using organ bath apparatus connected to a force transductor. Usually, the described...

23 September 2020 6,461 2 View

What systematic experiment will help determine whether or not a range of mutants with similar phenotype are derived from a common ancestor-cultivar?

Several soybean cultivars were mutagenized to produce many lines of M0, M1, M2 etc. In order to determine the genetic basis of an observed phenotype, an M0 level mutant was hybridized with its...

09 August 2020 9,224 3 View